Red grape extract with resveratrol, OPCs and anthocyanins made from French grapes.
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Anti-allergic activity of grapeseed extract

Anti-allergic activity of grapeseed extract
May 11, 2020 polyphine
In Uncategorized

Anti-allergic activity of grapeseed extract (GSE) on RBL-2H3 mast cells

Abstract

Grapeseed extract (GSE) is a rich source of natural phenolic compounds and possesses various pharmacological activities, including antioxidant, anticarcinogenic and anti-inflammatory properties. However, effects of GSE on immunoglobulin (Ig) E-mediated allergic responses still remain elusive. In the present study, the effects of GSE on activation and degranulation of RBL-2H3 mast cells were investigated. GSE pretreatments (20–100 μg/ml) reduced IgE-antigen mediated release of β-hexosaminidase and histamine in RBL-2H3 cells. Additionally, GSE reversibly inhibited expression of FcεRI on RBL-2H3 cells. GSE treatments caused a significant elevation of intracellular cAMP levels, whereas the Ca2+ influx upon antigen stimulation was inhibited. Suppression on FcεRI expression together with decreased calcium uptake and increased cAMP level might be involved in attenuated degranulation of mast cells by GSE treatment. Our results suggest a possible pharmaceutical application of GSE in treating type I allergic diseases.

Highlights

► GSE exhibits no cytotoxicity on RBL-2H3 mast cells. ► GSE inhibits degranulation of inflammatory mediators from activated mast cells. ► GSE reduces expression of high affinity IgE receptors on mast cells. ► GSE reduces calcium influx while increasing intracellular cAMP levels in mast cells

Abbreviations

BSA bovine serum albumin
cAMP cyclic adenosine monophosphate
DNP dinitrophenyl
FcεRI high-affinity IgE receptor
FITC fluorescein isothiocyanate
IgE immunoglobulin E
IL interleukin
mIgE-DNP murine anti-dinitrophenol IgE

Source: https://www.sciencedirect.com/science/article/abs/pii/S030881461101661X

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